Step 5: Methods Video Β πŸ§ͺ

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Find out how researchers narrowed down their search area for golden moles using a trained scent detection dog. Once the dog detected moles, the researchers collected environmental samples from that location and took them back into the lab for DNA extraction and sequencing.

So how exactly did they do this? Let’s get some methods. The researchers collected 49 eDNA samples by taking soil samples from six sites where there was golden mole activity, which they discovered using a trained scent detection dog. They also sampled DNA from two golden moles they captured in a pitfall trap and from two golden moles that were killed by people’s pets. Back in the lab they extracted and sequenced the DNA and eDNA samples that they had. The researchers focused on three sections of the DNA that had unique sequences for each species, like a barcode. Then they matched the eDNA with the DNA samples they collected and DNA samples from a database to figure out which golden mole species they came from.

Find out how researchers narrowed down their search area for golden moles using a trained scent detection dog. Once the dog detected moles, the researchers collected environmental samples from that location and took them back into the lab for DNA extraction and sequencing.

So how exactly did they do this? Let’s get some methods. The researchers collected 49 eDNA samples by taking soil samples from six sites where there was golden mole activity, which they discovered using a trained scent detection dog. They also sampled DNA from two golden moles they captured in a pitfall trap and from two golden moles that were killed by people’s pets. Back in the lab they extracted and sequenced the DNA and eDNA samples that they had. The researchers focused on three sections of the DNA that had unique sequences for each species, like a barcode. Then they matched the eDNA with the DNA samples they collected and DNA samples from a database to figure out which golden mole species they came from.

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